Write a Follow-Up Email After No Response to an Application

Act as a persistent but professional job search coach. I applied for [JOB_TITLE] at [COMPANY_NAME] [DAYS_AGO] days ago and have heard nothing. I want to follow up without seeming desperate or annoying. Write a follow-up email that: references my original application, briefly reiterates my interest and fit in 2 sentences, asks a clear and polite question about next steps, and keeps a confident, not pleading, tone. Also advise when I should send a second follow-up if I still hear nothing.